Blood spot screening and confirmatory tests for syphilis antibody

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Blood spot screening and confirmatory tests for syphilis antibody.

We developed a blood spot test for syphilis antibody using enzyme-linked immunosorbent assay (ELISA) technology. Dried blood was eluted by buffered saline or, for a supplementary confirmatory test, by treponemal-antibody test diluent. Eluates were diluted in an absorption buffer (Calypte Biomedical, Berkeley, Calif.) and added to plate wells coated with cardiolipin antigen (ADI Diagnostics, Tor...

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It is time to use treponema-specific antibody screening tests for diagnosis of syphilis.

Assays that detect treponema-specific antibodies, which are either automated or can be done as point-of-care tests, have been developed, some of which are FDA approved. These assays have the advantage of being easily performed and demonstrate high sensitivity, both key features of an infectious disease screening test. As a result, many high-volume clinical laboratories have begun to offer a rev...

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Two methods of performing serological screening tests for syphilis are compared. One consisted of the Venereal Diseases Reference Laboratory (VDRL) slide test, the cardiolipin Wassermann reaction (CWR), and the Reiter protein complement fixation test (RPCFT) performed manually; the other was a fully automated system using two Technicon AutoAnalyzers (AAII), one for the automated reagin test (AR...

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Relevance of molecular tests for HTLV-1 infection as confirmatory tests after the first sero-screening.

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1992

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.30.9.2353-2358.1992